Journal: Thoracic Cancer
Article Title: Effects of Autophagy Inhibition by SAR405 , a Selective VPS34 Inhibitor, on Pleural Mesothelioma Cells
doi: 10.1111/1759-7714.70255
Figure Lengend Snippet: Effects of SAR405 in a 3D spheroid model. (A) Spheroid cells were cultured for 72 h with 5.0–10.0 μM SAR405, or dimethyl sulfoxide (DMSO) as a control. Spheroids were imaged using a ZOE Fluorescent Cell Imager. Scale bars = 100 μm. (B) Spheroid size was quantified as projected area using ImageJ software version 1.54 g. Data are the mean ± SD of three independent experiments. *** p < 0.001 compared with control.
Article Snippet: Following an additional 72 h of incubation, spheroids were imaged using a ZOE Fluorescent Cell Imager (Bio‐Rad), and spheroid size was quantified as projected area using ImageJ software (version 1.54 g; National Institutes of Health, Bethesda, MD, USA) [ ].
Techniques: Cell Culture, Control, Software